| FSTL3

Follistatin-related protein 3 is a protein that is encoded by the FSTL3 gene.Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis.Diseases associated with FSTL3 include Chronic Leukemia. Among its related pathways are Signaling by TGFB family members and Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.2	78.1	0
OD	2.17	1.74	1.17	0.78	0.47	0.32	0.23	0.08
Corrected OD	2.09	1.66	1.09	0.7	0.39	0.24	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human FSTL3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human FSTL3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	89-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98